Article
Turnip yellow mosaic virus RNAs with anticodon loop substitutions that result in decreased valylation fail to replicate efficiently.
Journal of virology - 1 Jun 1991
Tsai C H, Dreher T W
Abstract excerpt
Single and multiple nucleotide substitutions have been introduced into the anticodon loop of the tRNA-like structure of turnip yellow mosaic virus (TYMV) genomic RNA. We studied the effects of these mutations on in vitro valylation and on replication in Chinese cabbage protoplasts and plants. Only those mutants capable of efficient and complete valylation showed efficient replication in protoplasts and gave rise...
Topics
- Anticodon
- Base Sequence
- Escherichia coli
- Gene Amplification
- Genes
- Kinetics
- Molecular Sequence Data
- Mosaic Viruses
- Mutation
- Nucleic Acid Conformation
- Phenotype