Article
Real time PCR strategy for the identification of major lineages of Trypanosoma cruzi directly in chronically infected human tissues.
International journal for parasitology - 1 Apr 2005
Freitas J M, Lages-Silva E, Crema E, Pena S D J, Macedo A M
Abstract excerpt
Two evolutionary lineages, called Trypanosoma cruzi I and II, have been identified in T. cruzi, the etiologic agent of human Chagas disease. Here, we describe a molecular strategy for direct genetic typing of these major groups of T. cruzi directly in human tissues. The protocol is based on heminested PCR amplification of the D7 region of the 24Salpha ribosomal DNA (rDNA), followed by identification of the...
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