Article
Simple methodology to directly genotype Trypanosoma cruzi discrete typing units in single and mixed infections from human blood samples.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 1 Sept 2016
Bontempi Iván A, Bizai María L, Ortiz Sylvia, Manattini Silvia, Fabbro Diana, Solari Aldo, Diez Cristina
Abstract excerpt
Different DNA markers to genotype Trypanosoma cruzi are now available. However, due to the low quantity of parasites present in biological samples, DNA markers with high copy number like kinetoplast minicircles are needed. The aim of this study was to complete a DNA assay called minicircle lineage specific-PCR (MLS-PCR) previously developed to genotype the T. cruzi DTUs TcV and TcVI, in order to genotype DTUs TcI...
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