Article
Identification of Trypanosoma cruzi Discrete Typing Units by Multiplex Real-Time PCR.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2026
Cura Carolina Inés, Ramirez Juan Carlos
Abstract excerpt
Trypanosoma cruzi isolates are classified into six discrete typing units (DTUs), TcI to TcVI, and TcBat. An accurate identification of T. cruzi genotype is critical for understanding its epidemiology, pathogenesis, and response to treatment. Several typing methodologies have been developed with variable levels of complexity, selectivity, and analytical sensitivity. This chapter describes a standardized multiplex...
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