Article
Involvement of the "linker" region between the exonuclease and polymerization domains of phi29 DNA polymerase in DNA and TP binding.
Gene - 28 Mar 2005
Truniger Verónica, Bonnin Ana, Lázaro José M, de Vega Miguel, Salas Margarita
Abstract excerpt
For several DNA-dependent DNA polymerases it has been shown that their synthetic and degradative activities are organized in two separated modules. The functional coordination required between them to accomplish successfully the replication process is provided by important contacts with the substrate contributed by residues coming from both modules. These domains are connected by a central "linker" region...
Topics
- Amino Acid Sequence
- Bacillus Phages
- Base Sequence
- Binding Sites
- Conserved Sequence
- DNA
- DNA-Directed DNA Polymerase
- Deoxyadenine Nucleotides
- Exonucleases
- Molecular Sequence Data
- Mutation
- Mutation, Missense
- Polymers
- Protein Binding