Article
Formation of a functionally active sodium-translocating hybrid F1F0 ATPase in Escherichia coli by homologous recombination.
European journal of biochemistry - 15 Dec 1993
Kaim G, Dimroth P
Abstract excerpt
A deletion mutant of Escherichia coli lacking the genes for ATPase subunits a, c, b, delta and part of the alpha subunit was transformed with a plasmid containing the corresponding genes of the sodium-translocating ATPase of Propionigenium modestum. The respective DNA fragment of P. modestum was integrated into the genome of the E. coli deletion mutant by site-specific homologous recombination. The sites of this...
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