Article
Formation of a functionally active sodium-translocating hybrid F1F0 ATPase in Escherichia coli by homologous recombination.
European journal of biochemistry - 15 Dec 1993
Kaim G, Dimroth P
Abstract excerpt
A deletion mutant of Escherichia coli lacking the genes for ATPase subunits a, c, b, delta and part of the alpha subunit was transformed with a plasmid containing the corresponding genes of the sodium-translocating ATPase of Propionigenium modestum. The respective DNA fragment of P. modestum was...
Topics
- Adenosine Triphosphatases
- Amino Acid Sequence
- Base Sequence
- Biological Transport, Active
- Cation Transport Proteins
- Chromosomes, Bacterial
- Cloning, Molecular
- Dicyclohexylcarbodiimide
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Gram-Negative Anaerobic Bacteria
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Proteolipids
- Proton-Translocating ATPases