Article
[Analysis of biallelic inactivation of the von Hippel-Lindau tumor suppressor gene VHL in patients of renal cell carcinoma patient].
Zhonghua wai ke za zhi [Chinese journal of surgery] - 15 Jan 2005
Liu Ning, Gong Kan, Zhang Ning, Guo Hong-feng, Na Xi, Wu Guan, Na Yan-qun
Abstract excerpt
OBJECTIVE: To investigate biallelic inactivation of the von Hippel-Lindau tumor suppressor gene (VHL) in patient of renal cell carcinoma (RCC) patient. METHODS: We extracted tumor and normal DNA from 41 RCC patients. Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing. Two single nucleotide polymorphism (SNP) sites located in VHL gene were...
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