Article
The structure of Apo R268A human aldose reductase: hinges and latches that control the kinetic mechanism.
Biochimica et biophysica acta - 15 May 2005
Bohren Kurt M, Brownlee June M, Milne Amy C, Gabbay Kenneth H, Harrison David H T
Abstract excerpt
Aldose reductase (AR) catalyzes the NADPH-dependent reduction of glucose and other sugars to their respective sugar alcohols. The NADP+/NADPH exchange is the rate-limiting step for this enzyme and contributes in varying degrees to the catalytic rates of other aldo-keto reductase superfamily enzymes. The mutation of Arg268 to alanine in human recombinant AR removes one of the ligands of the C2-phosphate of NADP+...
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