Mechanism of aldose reductase inhibition: binding of NADP+/NADPH and alrestatin-like inhibitors.

doi:10.1021/bi00189a019

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Article

Mechanism of aldose reductase inhibition: binding of NADP+/NADPH and alrestatin-like inhibitors.

Biochemistry - 14 Jun 1994

Ehrig T, Bohren K M, Prendergast F G, Gabbay K H

Abstract excerpt

Aldose reductase enfolds NADP+/NADPH via a complex loop mechanism, with cofactor exchange being the rate-limiting step for the overall reaction. This study measures the binding constants of these cofactors in the wild-type enzyme, as well as a variety of active-site mutants (C298A, Y48H, Y48F, Y2...

Topics

Aldehyde Reductase
Base Sequence
Binding Sites
Coenzymes
Isoquinolines
Molecular Sequence Data
Mutation
NADP
Oligodeoxyribonucleotides
Protein Binding
Recombinant Proteins
Spectrometry, Fluorescence
Tryptophan

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