Article
Variation in genomic Alu repeat density as a basis for rapid construction of low resolution physical maps of human chromosomes.
Chromosoma - 1 Mar 1992
Lane M J, Waterbury P G, Carroll W T, Smardon A M, Faldasz B D, Peshick S M, Mante S, Huckaby C S, Kouri R E, Hanlon D J
Abstract excerpt
Human DNA restriction fragments containing high numbers of Alu repeat sequences can be preferentially detected in the presence of other human DNA restriction fragments in DNA from human: rodent somatic cell hybrids when the DNA is fragmented with enzymes that cleave mammalian DNA infrequently. This ability to lower the observed human DNA complexity allowed us to develop an approach to order rapidly somatic hybrid...
Topics
- Chromosome Mapping
- Chromosomes, Human, Pair 17
- Genetic Variation
- Humans
- Hybrid Cells
- Karyotyping
- Nucleic Acid Hybridization
- Repetitive Sequences, Nucleic Acid