Article
[Quantitative analysis of human globin gene expression in beta-thalassemia using real-time RT-PCR].
Yi chuan = Hereditas - 1 Jan 2005
Han Jun-Ying, Zeng Rui-Ping, Cheng Gang, Hu Bin, Li Hu, Lai Yong-Rong
Abstract excerpt
Whole blood samples were collected from 100 normal healthy adults, from umbilical cord of 33 newborn infants, 111 individuals with beta-thalassemia minor (beta(T)/beta(A),alphaalpha/alphaalpha) and 39 with beta-thalassemia major (beta(T)/beta(T),alphaalpha/alphaalpha). Prior to quantitative analysis of globin gene expression, DNA was extracted from all blood samples and used for beta-thalassemia genotype...
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