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Cloning, biochemical and phylogenetic characterizations of gamma-glutamylcysteine synthetase from Anabaena sp. PCC 7120.

Plant & cell physiology - 1 Apr 2005

Ashida Hiroyuki, Sawa Yoshihiro, Shibata Hitoshi

Abstract excerpt

Gamma-glutamylcysteine synthetase (EC 6.3.2.2, gamma-GCS) catalyzes the first step of glutathione synthesis: l-Glu + l-Cys + ATP = gamma-l-glutamyl-l-cysteine (gamma-GC) + ADP + Pi. We have cloned the gene alr3351 of Anabaena sp. PCC 7120, expressed the recombinant enzyme in Escherichia coli, and characterized its product as gamma-GCS by analyzing gamma-GC production, ADP formation and Pi release. Apparent Km...

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PMID: 15695431
DOI: 10.1093/pcp/pci056

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