Article
Novel mutants of Erwinia carotovora subsp. carotovora defective in the production of plant cell wall degrading enzymes generated by Mu transpososome-mediated insertion mutagenesis.
FEMS microbiology letters - 1 Feb 2005
Laasik Eve, Ojarand Merli, Pajunen Maria, Savilahti Harri, Mäe Andres
Abstract excerpt
As in Erwinia carotovora subsp. carotovora the regulation details of the main virulence factors, encoding extracellular enzymes that degrade the plant cell wall, is only rudimentally understood, we performed a genetic screen to identify novel candidate genes involved in the process. Initially, we used Mu transpososome-mediated mutagenesis approach to generate a comprehensive transposon insertion mutant library of...
Topics
- Bacterial Proteins
- Bacteriophage mu
- Cell Wall
- DNA Transposable Elements
- Electroporation
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Pectobacterium carotovorum