Article
Localization of transposon insertions in pathogenicity mutants of Erwinia amylovora and their biochemical characterization.
Journal of general microbiology - 1 May 1992
Bellemann P, Geider K
Abstract excerpt
Transposon Tn5, on a mobilizable ColE1 plasmid, on a Ti plasmid derepressed for bacterial transfer, and on the bacteriophage fd genome, was used to construct pathogenicity mutants of the fire blight pathogen Erwinia amylovora. Eleven nonpathogenic mutants were isolated from 1600 independent mutants screened. These mutants were divided into three types: auxotrophs, exopolysaccharide (EPS)-deficient mutants and a...
Topics
- Chromosome Mapping
- DNA Transposable Elements
- Erwinia
- Fruit
- Genetic Complementation Test
- Genetic Vectors
- Mutagenesis, Insertional
- Phenotype
- Plant Diseases
- Polysaccharides
- Virulence
