Article
Characterization of transposon insertion out- mutants of Erwinia carotovora subsp. carotovora defective in enzyme export and of a DNA segment that complements out mutations in E. carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Erwinia chrysanthemi.
Journal of bacteriology - 1 Jun 1990
Murata H, Fons M, Chatterjee A, Collmer A, Chatterjee A K
Abstract excerpt
Soft-rotting Erwinia spp. export degradative enzymes to the cell exterior (Out+), a process contributing to their ability to macerate plant tissues. Transposon (Tn5, Tn10, Tn10-lacZ) insertion Out- mutants were obtained in Erwinia carotovora subsp. carotovora 71 by using plasmid and bacteriophage lambda delivery systems. In these mutants, pectate lyases, polygalacturonase, and cellulase, which are normally...
Topics
- Biological Transport
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Erwinia
- Genes, Bacterial
- Mutation
- Sequence Homology, Nucleic Acid