Article
Probing the DNA kink structure induced by the hyperthermophilic chromosomal protein Sac7d.
Nucleic acids research - 1 Jan 2005
Chen Chin-Yu, Ko Tzu-Ping, Lin Ting-Wan, Chou Chia-Cheng, Chen Chun-Jung, Wang Andrew H-J
Abstract excerpt
Sac7d, a small, abundant, sequence-general DNA-binding protein from the hyperthermophilic archaeon Sulfolobus acidocaldarius, causes a single-step sharp kink in DNA (approximately 60 degrees) via the intercalation of both Val26 and Met29. These two amino acids were systematically changed in size to probe their effects on DNA kinking. Eight crystal structures of five Sac7d mutant-DNA complexes have been analyzed....
Topics
- Archaeal Proteins
- Binding Sites
- Chromosomes, Archaeal
- DNA
- DNA-Binding Proteins
- Hot Temperature
- Models, Molecular
- Mutation
- Nucleic Acid Conformation