Article
Characteristics of the polyamine transporter TPO1 and regulation of its activity and cellular localization by phosphorylation.
The Journal of biological chemistry - 11 Mar 2005
Uemura Takeshi, Tachihara Ken, Tomitori Hideyuki, Kashiwagi Keiko, Igarashi Kazuei
Abstract excerpt
The subcellular localization of the polyamine transporter TPO1 of Saccharomyces cerevisiae was determined by sucrose gradient centrifugation and indirect immunofluorescence microscopy. When expressed from a multi-copy vector, TPO1 was located mainly on the plasma membrane, but with some localization on the vacuolar membrane. Polyamine transport by TPO1 was dependent on pH. Uptake of spermidine and spermine...
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