Article
Direct detection of isoniazid-resistant Mycobacterium tuberculosis in respiratory specimens by multiplex allele-specific polymerase chain reaction.
Diagnostic microbiology and infectious disease - 1 Jan 2011
Siu Gilman Kit Hang, Tam Yuk Ho, Ho Pak Leung, Lee Ann Siew Gek, Que Tak Lun, Tse Cindy Wing Sze, Yip Kam Tong, Lam Jason Tsz Hin, Cheng Vincent Chi Chung, Yuen Kwok Yung, Yam Wing Cheong
Abstract excerpt
This study evaluated the feasibility of using 2 multiplex allele-specific polymerase chain reaction (MAS-PCR) assays targeting 2 mutations (codon 315 of the katG gene and the 15th nucleotide preceding the mabA-inhA operon) to directly detect isoniazid (INH)-resistant Mycobacterium tuberculosis in cultured isolates and respiratory specimens. A total of 203 M. tuberculosis isolates and 487 respiratory specimens...
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