Article
AlphaA-crystallin interacting regions in the small heat shock protein, alphaB-crystallin.
Biochemistry - 21 Dec 2004
Sreelakshmi Yellamaraju, Santhoshkumar Puttur, Bhattacharyya Jaya, Sharma K Krishna
Abstract excerpt
Amino acid sequences of alphaB-crystallin, involved in interaction with alphaA-crystallin, were determined by using peptide scans. Positionally addressable 20-mer overlapping peptides, representing the entire sequence of alphaB-crystallin, were synthesized on a PVDF membrane. The membrane was blocked with albumin and incubated with purified alphaA-crystallin. Probing the membrane with alphaA-crystallin-specific...
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