Article
rna12+, a gene of Saccharomyces cerevisiae involved in pre-rRNA maturation. Characterization of a temperature-sensitive mutant, cloning and sequencing of the gene.
Molecular & general genetics : MGG - 1 Mar 1992
Liang S, Alksne L, Warner J R, Lacroute F
Abstract excerpt
RNA12-1 is a dominant temperature-sensitive (Ts) yeast mutant which has previously been reported to exhibit a defect in RNA accumulation at 37 degrees C. We further characterized this mutant through analyses of rRNA transcription rates and maturation. The results show that pre-rRNA is normally sy...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Chromosomes, Fungal
- Cloning, Molecular
- Genes, Fungal
- Hot Temperature
- Methylation
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- RNA Polymerase II
- RNA Precursors
- RNA Processing, Post-Transcriptional
- RNA, Ribosomal
- Restriction Mapping
- Saccharomyces cerevisiae
- Transcription, Genetic