Article
[Comparative analysis of serologic typing and HLA-II typing by micro-PCR-SSP].
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA - 1 Mar 2002
Wu Da-Lin, Ling Han-Xin, Ding Hong, Zhang Yi
Abstract excerpt
OBJECTIVE: To evaluate the accuracy of polymerase chain reaction with sequence specific primers (PCR-SSP) in HLA-II genotyping and analyze the causes of the errors occurring during the genotyping. METHOD: Blood samples were obtained form patients with chronic renal insufficiency, leukemia or thalassemia and also from normal subjects. HLA-DR and -DQ genotyping of the sera from the 110 subjects was performed using...
Topics
- Alleles
- Antibodies, Monoclonal
- DNA
- Gene Frequency
- Genotype
- HLA-DQ Antigens
- HLA-DR Antigens
- Histocompatibility Antigens Class II
- Histocompatibility Testing
- Humans
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive
- Polymerase Chain Reaction
