Article
[Comparative analysis of serologic typing and HLA-II typing by micro-PCR-SSP].
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA - 1 Mar 2002
Wu Da-Lin, Ling Han-Xin, Ding Hong, Zhang Yi
Abstract excerpt
OBJECTIVE: To evaluate the accuracy of polymerase chain reaction with sequence specific primers (PCR-SSP) in HLA-II genotyping and analyze the causes of the errors occurring during the genotyping. METHOD: Blood samples were obtained form patients with chronic renal insufficiency, leukemia or thalassemia and also from normal subjects. HLA-DR and -DQ genotyping of the sera from the 110 subjects was performed using...
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