Article
Insertion of a classical nuclear import signal into the matrix domain of the Rous sarcoma virus Gag protein interferes with virus replication.
Journal of virology - 1 Dec 2004
Garbitt Rachel A, Bone Karen R, Parent Leslie J
Abstract excerpt
The Rous sarcoma virus Gag protein undergoes transient nuclear trafficking during virus assembly. Nuclear import is mediated by a nuclear targeting sequence within the MA domain. To gain insight into the role of nuclear transport in replication, we investigated whether addition of a "classical " nuclear localization signal (NLS) in Gag would affect virus assembly or infectivity. A bipartite NLS derived from...
Topics
- Active Transport, Cell Nucleus
- Amino Acid Sequence
- Animals
- Avian Sarcoma Viruses
- Cell Nucleus
- Cells, Cultured
- Gene Expression Regulation, Viral
- Gene Products, gag
- Green Fluorescent Proteins
- Molecular Sequence Data
- Mutation
- Nuclear Localization Signals
- Quail