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Neutrophil microbicidal activity: screening bacterial mutants for survival after phagocytosis using quantitative PCR.

Japanese journal of infectious diseases - 1 Oct 2004

Rosen Henry, Lewis Patrick J, Nitzel Cory M L

Abstract excerpt

When a constant gene replacement sequence is introduced into bacteria to produce mutants and the flanking chromosomal sequences are known, it is possible to use a quantitative polymerase chain reaction method (QPCR) to compare the concurrent survival of the different bacterial mutants under identical conditions. We describe Escherichia coli survival following neutrophil phagocytosis among three mutants deleted...

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PMID: 15507758

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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