Reversion of Q beta RNA phage mutants by homologous RNA recombination.

doi:10.1128/JVI.66.4.2435-2442.1992

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Article

Reversion of Q beta RNA phage mutants by homologous RNA recombination.

Journal of virology - 1 Apr 1992

Palasingam K, Shaklee P N

Abstract excerpt

Q beta phage RNAs with inactivating insertion (8-base) or deletion (17-base) mutations within their replicase genes were prepared from modified Q beta cDNAs and transfected into Escherichia coli spheroplasts containing Q beta replicase provided in trans by a resident plasmid. Replicase-defective...

Topics

Amino Acid Sequence
Base Sequence
Coliphages
DNA, Viral
Escherichia coli
Genetic Complementation Test
Molecular Sequence Data
Mutation
Q beta Replicase
RNA, Viral
Recombination, Genetic

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