Article
Reversion of Q beta RNA phage mutants by homologous RNA recombination.
Journal of virology - 1 Apr 1992
Palasingam K, Shaklee P N
Abstract excerpt
Q beta phage RNAs with inactivating insertion (8-base) or deletion (17-base) mutations within their replicase genes were prepared from modified Q beta cDNAs and transfected into Escherichia coli spheroplasts containing Q beta replicase provided in trans by a resident plasmid. Replicase-defective (Rep-) Q beta phage produced by these spheroplasts were detected as normal-sized plaques on lawns of cells containing...
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