Article
Interference with viral infection by RNA replicase deleted at the carboxy-terminal region.
Journal of biochemistry - 1 Jul 1990
Inokuchi Y, Hirashima A
Abstract excerpt
Escherichia coli cells harboring an altered Q beta RNA replicase which has amino acid substitutions of the glycine residue at position 357 in the conserved sequence Tyr356-Gly357-Asp358-Asp359 of the beta-subunit protein lost the replicase activity but interfered with proliferation of Q beta phage [Inokuchi and Hirashima (1987) J. Virol. 61, 3946-3949]. To examine the mechanism of the interference, we further...
Topics
- Alanine
- Amino Acid Sequence
- Bacterial Proteins
- Bacteriophage lambda
- Coliphages
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genes, Viral
- Molecular Sequence Data
- Mutation
- RNA-Dependent RNA Polymerase