Article
Human p53 and CDC2Hs genes combine to inhibit the proliferation of Saccharomyces cerevisiae.
Molecular and cellular biology - 1 Mar 1992
Nigro J M, Sikorski R, Reed S I, Vogelstein B
Abstract excerpt
Human wild-type and mutant p53 genes were expressed under the control of a galactose-inducible promoter in Saccharomyces cerevisiae. The growth rate of the yeast was reduced in cells expressing wild-type p53, whereas cells transformed with mutant p53 genes derived from human tumors were less affe...
Topics
- Base Sequence
- Blotting, Western
- CDC2 Protein Kinase
- Cloning, Molecular
- DNA
- Electrophoresis, Gel, Two-Dimensional
- Flow Cytometry
- Humans
- Molecular Sequence Data
- Peptide Mapping
- Phenotype
- Phosphorylation
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- Saccharomyces cerevisiae
- Transformation, Genetic
- Tumor Suppressor Protein p53