Article
Relationship of primary structure to functional properties of the cytotoxin protein from Pseudomonas aeruginosa.
Naunyn-Schmiedeberg's archives of pharmacology - 1 May 1991
Xiong G M, Struckmeier M, Alberti U, Lutz F
Abstract excerpt
Deletion mutants of plasmid pSN3 carrying the Pseudomonas aeruginosa cytotoxin gene were prepared and expression in Escherichia coli of proteins with different molecular weights has been proved by Western blot. In addition, through PCR amplification of the cytotoxin gene and ligation into the vector, a single nucleotide change leading to an amino acid exchange from histidine to arginine has been constructed. The...
Topics
- Animals
- Bacterial Proteins
- Blotting, Western
- Cytotoxins
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genetic Vectors
- Mutation
- Pseudomonas aeruginosa
- Rabbits
- Structure-Activity Relationship