Article
Negative regulators of the PHO system of Saccharomyces cerevisiae: characterization of PHO80 and PHO85.
Molecular & general genetics : MGG - 1 Feb 1992
Uesono Y, Tokai M, Tanaka K, Tohe A
Abstract excerpt
Both PHO80 and PHO85 genes are required to establish the repressed state of the PHO system of Saccharomyces cerevisiae. S1 nuclease protection analysis of the PHO85 transcript revealed that the PHO85 gene contains an intron at the 6th codon of the gene. Each of the fusion proteins, LacZ-Pho80 and LacZ-Pho85, was produced into Escherichia coli and used as an antigen to raise antibodies in a rabbit. Using the...
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