Article
Synthetic growth phenotypes of Escherichia coli lacking ppGpp and transketolase A (tktA) are due to ppGpp-mediated transcriptional regulation of tktB.
Molecular microbiology - 1 Aug 2008
Harinarayanan Rajendran, Murphy Helen, Cashel Michael
Abstract excerpt
Many physiological adjustments to nutrient changes involve ppGpp. Recent attempts to deduce ppGpp regulatory effects using proteomics or gene profiling can rigorously identify proteins or transcripts, but the functional significance is often unclear. Using a random screen for synthetic lethals we found a ppGpp-dependent functional pathway that operates through transketolase B (TktB), and which is 'buffered' in...
Topics
- Alleles
- Bacterial Proteins
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Guanosine Tetraphosphate
- INDEL Mutation
- Phenotype
- Sigma Factor