Article
Assembly and targeting of peripheral and integral membrane subunits of the yeast vacuolar H(+)-ATPase.
The Journal of biological chemistry - 5 Jan 1992
Kane P M, Kuehn M C, Howald-Stevenson I, Stevens T H
Abstract excerpt
Previous purification and characterization of the yeast vacuolar proton-translocating ATPase (H(+)-ATPase) have indicated that it is a multisubunit complex consisting of both integral and peripheral membrane subunits (Uchida, E., Ohsumi, Y., and Anraku, Y. (1985) J. Biol. Chem. 260, 1090-1095; Kane, P. M., Yamashiro, C. T., and Stevens, T. H. (1989) J. Biol. Chem. 264, 19236-19244). We have obtained monoclonal...
Topics
- Antibodies, Monoclonal
- Base Sequence
- Biological Transport
- Blotting, Western
- Carbonates
- Electrophoresis, Polyacrylamide Gel
- Fluorescent Antibody Technique
- Genes, Fungal
- Hydrogen-Ion Concentration
- Membrane Proteins
