Article
Mutational analysis of the catalytic subunit of the yeast vacuolar proton-translocating ATPase.
Biochemistry - 20 Aug 1996
Liu J, Kane P M
Abstract excerpt
In order to generate a set of tools for probing structure-function relationships in the catalytic subunit of the yeast vacuolar H(+)-ATPase, the gene encoding this subunit (VMA1) was randomly mutagenized. Mutant plasmids unable to complement the growth defects of yeast cells lacking an intact VMA1 gene were isolated and sequenced. Eight different mutant alleles of VMA1 were examined for levels of the catalytic...
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