Mutational analysis of the catalytic subunit of the yeast vacuolar proton-translocating ATPase.

doi:10.1021/bi9608065

Back to search

Article

Mutational analysis of the catalytic subunit of the yeast vacuolar proton-translocating ATPase.

Biochemistry - 20 Aug 1996

Liu J, Kane P M

Abstract excerpt

In order to generate a set of tools for probing structure-function relationships in the catalytic subunit of the yeast vacuolar H(+)-ATPase, the gene encoding this subunit (VMA1) was randomly mutagenized. Mutant plasmids unable to complement the growth defects of yeast cells lacking an intact VMA...

Topics

Alleles
Base Sequence
Catalysis
DNA Primers
Molecular Sequence Data
Mutagenesis
Plasmids
Proton-Translocating ATPases
Protons
Saccharomyces cerevisiae
Structure-Activity Relationship
Vacuoles

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.