Article
Pinpoint mapping of recognition residues on the cohesin surface by progressive homologue swapping.
The Journal of biological chemistry - 8 Oct 2004
Nakar David, Handelsman Tal, Shoham Yuval, Fierobe Henri-Pierre, Belaich Jean-Pierre, Morag Ely, Lamed Raphael, Bayer Edward A
Abstract excerpt
The high affinity cohesin-dockerin interaction dictates the suprastructural assembly of the multienzyme cellulosome complex. The connection between affinity and species specificity was studied by exploring the recognition properties of two structurally related cohesin species of divergent specificity. The cohesins were examined by progressive rounds of swapping, in which corresponding homologous stretches were...
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