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Article

Crucial roles of single residues in binding affinity, specificity, and promiscuity in the cellulosomal cohesin-dockerin interface.

The Journal of biological chemistry - 29 May 2015

Slutzki Michal, Reshef Dan, Barak Yoav, Haimovitz Rachel, Rotem-Bamberger Shahar, Lamed Raphael, Bayer Edward A, Schueler-Furman Ora

Abstract excerpt

Interactions between cohesin and dockerin modules play a crucial role in the assembly of multienzyme cellulosome complexes. Although intraspecies cohesin and dockerin modules bind in general with high affinity but indiscriminately, cross-species binding is rare. Here, we combined ELISA-based experiments with Rosetta-based computational design to evaluate the contribution of distinct residues at the Clostridium...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25833947
DOI: 10.1074/jbc.M115.651208
PMCID: PMC4447945

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