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Article

High-throughput screening of cohesin mutant libraries on cellulose microarrays.

Methods in enzymology - 1 Jan 2012

Slutzki Michal, Ruimy Vered, Morag Ely, Barak Yoav, Haimovitz Rachel, Lamed Raphael, Bayer Edward A

Abstract excerpt

The specificity of cohesin-dockerin interactions is critically important for the assembly of cellulosomal enzymes into the multienzyme cellulolytic complex (cellulosome). In order to investigate the origins of the observed specificity, a variety of selected amino acid positions at the cohesin-dockerin interface can be subjected to mutagenesis, and a library of mutants can be constructed. In this chapter, we...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 22608741
DOI: 10.1016/B978-0-12-415931-0.00024-0

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