Article
Identification of two new genes involved in twitching motility in Pseudomonas aeruginosa.
Microbiology (Reading, England) - 1 Aug 2004
Shan Zhiying, Xu Haijin, Shi Xingqi, Yu Yan, Yao Hongming, Zhang Xiuming, Bai Yanling, Gao Caichang, Saris Per E J, Qiao Mingqiang
Abstract excerpt
Mu transposition complexes were used for transposon mutagenesis of Pseudomonas aeruginosa strain PA68. Mu DNA transposition complexes were assembled with MuA transposase and an artificial mini-Mu transposon in vitro, and introduced into Pseudomonas aeruginosa by electroporation. Eight mutants deficient in twitching motility were isolated. Southern blotting confirmed that the insertions had occurred as single...
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