Article
Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic gamma-tubulin complexes.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Apr 2006
Masuda Hirohisa, Toda Takashi, Miyamoto Rumi, Haraguchi Tokuko, Hiraoka Yasushi
Abstract excerpt
The gamma-tubulin complex acts as a nucleation unit for microtubule assembly. It remains unknown, however, how spatial and temporal regulation of the complex activity affects microtubule-mediated cellular processes. Alp4 is one of the essential components of the S. pombe gamma-tubulin complex. We show here that overproduction of a carboxy-terminal form of Alp4 (Alp4C) and its derivatives tagged to a nuclear...
Topics
- Cell Cycle Proteins
- Cell Division
- Cell Nucleus
- Cells, Cultured
- Cytoplasm
- G2 Phase
- Microtubule-Associated Proteins
- Microtubules
- Mutation
- Nuclear Localization Signals
- Nuclear Proteins
- Phenotype
- Protein-Tyrosine Kinases