Article
Bioreactor-based bone tissue engineering: the influence of dynamic flow on osteoblast phenotypic expression and matrix mineralization.
Proceedings of the National Academy of Sciences of the United States of America - 3 Aug 2004
Yu Xiaojun, Botchwey Edward A, Levine Elliot M, Pollack Solomon R, Laurencin Cato T
Abstract excerpt
An important issue in tissue engineering concerns the possibility of limited tissue ingrowth in tissue-engineered constructs because of insufficient nutrient transport. We report a dynamic flow culture system using high-aspect-ratio vessel rotating bioreactors and 3D scaffolds for culturing rat calvarial osteoblast cells. 3D scaffolds were designed by mixing lighter-than-water (density, <1g/ml) and...
Topics
- Alkaline Phosphatase
- Animals
- Biomarkers
- Bioreactors
- Calcification, Physiologic
- Cell Culture Techniques
- Cell Division
- Osteoblasts
- Osteocalcin
- Osteopontin
- Perfusion
- Phenotype
- Rats