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Article

Mineralized matrix deposition by marrow stromal osteoblasts in 3D perfusion culture increases with increasing fluid shear forces.

Proceedings of the National Academy of Sciences of the United States of America - 9 Dec 2003

Sikavitsas Vassilios I, Bancroft Gregory N, Holtorf Heidi L, Jansen John A, Mikos Antonios G

Abstract excerpt

In this study we report on direct involvement of fluid shear stresses on the osteoblastic differentiation of marrow stromal cells. Rat bone marrow stromal cells were seeded in 3D porous titanium fiber mesh scaffolds and cultured for 16 days in a flow perfusion bioreactor with perfusing culture media of different viscosities while maintaining the fluid flow rate constant. This methodology allowed exposure of the...

Topics

Alkaline Phosphatase
Animals
Bone Marrow Cells
Calcium
Cells, Cultured
Culture Media
DNA
Extracellular Matrix
Male
Microscopy, Electron, Scanning

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Osteoblasts

Perfusion

Phenotype

Rats

Rats, Wistar

Stress, Mechanical

Stromal Cells

Time Factors

Source

Open in PubMed Open full text

Identifiers

PMID: 14657343
DOI: 10.1073/pnas.2434367100
PMCID: PMC299759

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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