Article
Structure of the I1 early intermediate of photoactive yellow protein by FTIR spectroscopy.
Nature structural biology - 1 Mar 2001
Brudler R, Rammelsberg R, Woo T T, Getzoff E D, Gerwert K
Abstract excerpt
To understand how proteins translate the energy of sunlight into defined conformational changes, we have measured the photocycle reactions of photoactive yellow protein (PYP) using time-resolved step scan Fourier transform infrared (FTIR) spectroscopy. Global fit analysis yielded the same apparent time constants for the reactions of the chromophore, the protonation changes of protein side chains and the protein...
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