Article
Stable high-level transgene expression in Arabidopsis thaliana using gene silencing mutants and matrix attachment regions.
The Plant journal : for cell and molecular biology - 1 Aug 2004
Butaye Katleen M J, Goderis Inge J W M, Wouters Piet F J, Pues Jonathan M-T G, Delauré Stijn L, Broekaert Willem F, Depicker Ann, Cammue Bruno P A, De Bolle Miguel F C
Abstract excerpt
Basic and applied research involving transgenic plants often requires consistent high-level expression of transgenes. However, high inter-transformant variability of transgene expression caused by various phenomena, including gene silencing, is frequently observed. Here, we show that stable, high-level transgene expression is obtained using Arabidopsis thaliana post-transcriptional gene silencing (PTGS) sgs2 and...
Topics
- Arabidopsis
- DNA, Bacterial
- Gene Expression
- Genes, Plant
- Genes, Reporter
- Glucuronidase
- Matrix Attachment Regions
- Mutation
- Phenotype
- Plants, Genetically Modified