Article
Establishment of an efficient virus-induced gene silencing (VIGS) assay in Arabidopsis by Agrobacterium-mediated rubbing infection.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Manhães Ana Marcia E de A, de Oliveira Marcos V V, Shan Libo
Abstract excerpt
Several VIGS protocols have been established for high-throughput functional genomic screens as it bypasses the time-consuming and laborious process of generation of transgenic plants. The silencing efficiency in this approach is largely hindered by a technically demanding step in which the first pair of newly emerged true leaves at the 2-week-old stage are infiltrated with a needleless syringe. To further...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.