SVISS - a novel transient gene silencing system for gene function discovery and validation in tobacco plants.

doi:10.1046/j.1365-313x.2002.01471.x

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Article

SVISS - a novel transient gene silencing system for gene function discovery and validation in tobacco plants.

The Plant journal : for cell and molecular biology - 1 Dec 2002

Gosselé Véronique, Faché Ina, Meulewaeter Frank, Cornelissen Marc, Metzlaff Michael

Abstract excerpt

We developed a novel, two-component transient gene silencing system in which the satellite tobacco mosaic virus (STMV) is used as vector for the delivery of inhibitory RNA into tobacco plants and the tobacco mosaic virus strain U2 (TMV-U2) is used as helper virus for supplying replication and movement proteins in trans. The main advantage of the system is that by uncoupling virus replication components from...

Topics

Gene Expression Regulation, Plant
Gene Silencing
Genes, Plant
Genetic Vectors
Molecular Sequence Data
Phenotype
Plant Leaves
RNA, Plant
Nicotiana
Tobacco mosaic satellite virus

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Open in PubMed

Identifiers

PMID: 12472699
DOI: 10.1046/j.1365-313x.2002.01471.x

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