Article
A new automated method for phenotyping arylesterase (EC 3.1.1.2) based upon inhibition of enzymatic hydrolysis of 4-nitrophenyl acetate by phenyl acetate.
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies - 1 Jul 1992
Haagen L, Brock A
Abstract excerpt
A new method for phenotyping human serum arylesterase (EC 3.1.1.2) is described and evaluated. The aromatic esters, phenyl acetate and 4-nitrophenyl acetate, were compared as substrates for spectrophotometric measurement of arylesterase activity. A method for arylesterase phenotyping, based upon inhibition of the enzymatic hydrolysis of 4-nitrophenyl acetate by phenyl acetate, was developed. The method was...
Topics
- Alleles
- Blood Donors
- Carboxylic Ester Hydrolases
- Humans
- Hydrolysis
- Isoenzymes
- Kinetics
- Nitrophenols
- Phenotype
- Phenylacetates
- Substrate Specificity