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Article

Toxicity-based selection of Escherichia coli mutants for functional recombinant protein production: application to an antibody fragment.

Protein engineering, design & selection : PEDS - 1 May 2004

Belin P, Dassa J, Drevet P, Lajeunesse E, Savatier A, Boulain J-C, Ménez A

Abstract excerpt

We propose a novel approach to the selection of Escherichia coli bacterial strains improved for the production of recombinant functional proteins. This approach is based on aggregation-induced toxicity of recombinant proteins. We show that selection of clones displaying a reduced toxicity is an efficient means of isolating bacteria producing recombinant protein with reduced aggregation in favour of correct...

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PMID: 15252207
DOI: 10.1093/protein/gzh054

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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