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Article

Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR.

BMC biotechnology - 13 Jul 2004

Bubner Ben, Gase Klaus, Baldwin Ian T

Abstract excerpt

BACKGROUND: After transformation, plants that are homozygous and contain one copy of the transgene are typically selected for further study. If real-time PCR is to be used to determine copy number and zygosity, it must be able to distinguish hemizygous from homozygous and one-copy from two-copy plants. That is, it must be able to detect two-fold differences. RESULTS: When transgenic Nicotiana attenuata plants...

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Identifiers

PMID: 15251044
DOI: 10.1186/1472-6750-4-14
PMCID: PMC493272

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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