Article
Reversion-reporter transgenes to analyze all six base-substitution pathways in Arabidopsis.
Plant physiology - 1 Mar 2011
Bollmann Stephanie R, Tominey Colin M, Hoffman Peter D, Hoffman Taylor M C, Hays John B
Abstract excerpt
To expand the repertoire of Arabidopsis (Arabidopsis thaliana) mutation-reporter transgenes, we constructed six mutant alleles in the same codon of the β-glucuronidase-encoding GUS transgene. Each allele reverts to GUS+ only via a particular one of the six transition/transversion pathways. AcV5 epitope tags, fused carboxyl terminal to the inactive GUS- proteins, enabled semiquantitative immunoassays in plant...
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