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Article

Accurate determination of zygosity in transgenic rice by real-time PCR does not require standard curves or efficiency correction.

Transgenic research - 1 Apr 2006

Prior Fiona A, Tackaberry Eilleen S, Aubin Rémy A, Casley William L

Abstract excerpt

A number of quantitative, real-time PCR methods have been developed for determining transgene copy numbers in plants. Here, we demonstrate that the Roche LightCycler system can be used to determine the zygosity of transgenic lines without the use of standard curves or efficiency correction calculations. We have developed a duplex PCR assay which permits the determination of zygosity, relative to a calibrator...

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PMID: 16604466
DOI: 10.1007/s11248-005-4024-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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