A Human Papilloma Virus Testing Algorithm Comprising a Combination of the L1 Broad-Spectrum SPF ₁₀ PCR Assay and a Novel E6 High-Risk Multiplex Type-Specific Genotyping PCR Assay

Abstract excerpt

Human papillomavirus (HPV) epidemiological and vaccine studies require highly sensitive HPV detection and genotyping systems. To improve HPV detection by PCR, the broad-spectrum L1-based SPF10 PCR DNA enzyme immunoassay (DEIA) LiPA system and a novel E6-based multiplex type-specific system (MPTS123) that uses Luminex xMAP technology were combined into a new testing algorithm. To evaluate this algorithm, cervical...

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