Article
The activity of a single-stranded promoter of plasmid ColIb-P9 depends on its secondary structure.
Molecular microbiology - 1 Jul 2004
Nasim M T, Eperon I C, Wilkins B M, Brammar W J
Abstract excerpt
The leading region of the conjugal bacterial plasmid ColIb-P9 contains three dispersed repeats of a 328 bp sequence homologous to Frpo, a sequence from plasmid F that acts as a promoter in single-stranded DNA. One of these sequences, ssi3, inactive in the double-stranded form, promoted in vitro transcription exclusively from the single strand that is transferred during conjugation. Promoter activity was dependent...
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