Article
Recombinant Escherichia coli as a gene delivery vector into airway epithelial cells.
Journal of controlled release : official journal of the Controlled Release Society - 18 Jun 2004
Fajac I, Grosse S, Collombet J-M, Thevenot G, Goussard S, Danel C, Grillot-Courvalin C
Abstract excerpt
To transfer genes into airway epithelial cells, we have generated auxotrophic dap Escherichia coli BM2710 mutant that expresses the invasin of Yersinia pseudotuberculosis and the listeriolysin of Listeria monocytogenes. E. coli BM2710 harboring a plasmid carrying the gfp gene was incubated with immortalized normal or cystic fibrosis (CF) airway epithelial cells or with primary bronchial epithelial cells grown as...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
